The Corneal Micropocket Assay

Angiogenesis can be assessed in a number of ways.  One method we commonly use in the lab is the corneal micropocket assay. This assay involves the implantation of a pellet containing growth factor into a mouse eye to stimulate blood vessels to grow in the otherwise avascular cornea.  This growth can be quantified and used to evaluate the pro- or anti-angiogenic ability of drugs as well as to compare the angiogenic potential of different strains of mice to help elucidate underlying genetic mechanisms of response. The video below is a step-by-step narrative of the assay as published in the Journal of Visualized Experiments in 2014.

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye33.06 MB